Nettet6. jul. 2024 · The Hoechst stains are a group of blue-fluorescent DNA probes. They have been used to stain DNA as early as the 1970s. Developed by the German company Hoechst AG, these dyes offer a … NettetApoptosis, a genetically programmed cellular event leads to biochemical and morphological changes in cells. Alterations in DNA caused by several factors affect …
Protocol: Staining Cells with Hoechst or DAPI Nuclear Stains
Nettet10. jan. 2024 · Hoechst stains are also cell permeable and can therefore be used in fixed and living cells. They exhibit lower toxicity than DAPI. A membrane-impermeable DNA stain is Propidium-Iodide which is often used to differentiate between living and dead cells in a cell culture because it cannot enter an intact cell. Nettet9. mai 2024 · To examine the membranolytic activities of peptides, the PI/Hoechst 33342 staining was performed in this study. The PI dye was used to stain damaged/dead cells, and Hoechst 33342 was used to specifically stain the nuclei of living cells [21,24]. PC 9 cells were seeded at 20,000 cells/well in a RPMI medium and allowed to adhere for 48 h. fresh foam 1080v9 women\u0027s
Staining cells with DAPI or Hoechst - University of Southern …
NettetHoechst dyes are cell- permeable and can bind to DNA in live or fixed cells. Thus, these stains are often called supravital, meaning that live cells survive a treatment with these … NettetCell death was determined by staining cells with Annexin V-allophycocyanin (APC) (AnnV, 0.1 μg/mL; BD Biosciences Pharmingen, San Jose, CA, USA) and propidium iodide ... To further discard nuclear alterations, we stained HeLa and MCF-7 cells with Hoechst, and nuclear morphology was assessed using a fluorescence microscope. HeLa ... NettetYes, Hoechst 33342 can stain dead cells, however Hoechst 33358 is the preferred dye that’s used for staining dead or fixed cells. Hoechst 33342 is generally used for staining live cells. Hoechst dyes are a fluorescent stains that … fresh foam 1080 z11 ・ aries