Web1 jan. 2009 · A three-plasmid yeast expression system utilizing the portable small ubiquitin-like modifier (SUMO) vector set combined with the efficient endogenous … Web16 jan. 2024 · S. cerevisiae INVSc1 (MATa/MATα his3 1 leu2 trp1-289 ura3-52) and S. cerevisiae CEN.PK2-1C (MATa; ura3-52; trp1-289; Leu2-3,112; his3Δ1; MAL2-8C; SUC2) were used as hosts. The BMMY medium composed of 10 g/L yeast extract, 20 g/L peptone and 20 g/L galactose was used for culturing yeast containing the inducible promoter.
Identification and characterization of an efficient acyl-CoA ...
Web我们提供INVSc1酵母株。这是一种二倍体酵母株,仅作表达用途。该酵母株不能形成良好的孢子,因此不适用于酵母遗传研究。INVSc1酵母株的基因型和表型如下: 基因 … WebThe yeast strain INVSc1 transformed with the plasmids carrying genome sequence of pat1 (Pat1-wt), carrying codon optimized sequence of pat1 (Pat1-co) or carrying mutation at … chip maker arizona
Saccharomyces Cerevisiae Strain Invsc1 Thermo Fisher Bioz
WebInvitrogen™ INV Sc 1, S. cerevisiae Yeast Strain. Fast-growing diploid strain excellent for expression. Manufacturer: Invitrogen™ C81000. Catalog No. C81000. $306.00 / Each of … WebPlease note that INVSc1 is a diploid strain that is auxotrophic for histidine, leucine, tryptophan, and uracil. The strain will not grow in SC minimal medium that is deficient in histidine, leucine, tryptophan, and uracil. A recipe for preparation of SC minimal medium is provided in the Appendix (see page 9). Initiating INVSc1 Culture Web5 jan. 2024 · The reconstructed plasmids and empty plasmid were then transformed into yeast strain INVSc1 using lithium acetate-polyethylene glycol-mediated transformation procedure. The positive transgenic clones were screened and cultivated in yeast synthetic drop-out medium (SD/-Ura) with 2% ( w/v ) glucose at 30 °C for 12 h. chipmaker arm